PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN

Purification and Molecular Weight Determination of Clostridium Botulinum Type E Toxin.

Gerwing, Julia (The University of British Columbia, Vancouver, British Columbia, Canada), Claude E. Dolman, M. E. Reichmann, and Hardial S. Bains. Purification and molecular weight determination of Clostridium botulinum type E toxin. J. Bacteriol. 88:216-219. 1964.-A method was developed whereby type E botulinus toxin can be obtained in a highly purified state by elution through acidified dieth...

Purification and activation of Clostridium botulinum type E toxin.

Type E strains of Clostridium botulinum tend to produce toxin of relatively low mouse killing power (administered intraperitoneally) in relation to its high lethality per os for man. The explanation of this peculiarity seems to lie in the phenomenon of "activation," whereby type E toxin, when exposed at an appropriate pH in vitro to certain bacterial enzymes (Dolman, 1953, 1957a; Sakaguchi and ...

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Purification and properties of Clostridium botulinum type F toxin.

Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed on diethylaminoethyl-cellulose at pH 6,0, O-(carboxymethyl) cellulose at pH 4.9, and finally diethylaminoethyl-cellulose at pH 8.1. The procedure recovered 14 percent of the toxin assayed in the star...

Purification and crystallization of Clostridium botulinum type C toxin.